Validated Rp-hplc Method for the Estimation of Telmisartan in Serum Samples
نویسندگان
چکیده
A simple, selective, linear, precise and accurate RP-HPLC method was developed and validated for rapid assay of telmisartan in serum samples. Isocratic elution at a flow rate of 1.0ml/min was employed on a Equsil, 250 X 4.6mm, 5μ at ambient temperature. The mobile phase consisted of buffer: Acetonitrile (35:65) (V/V). The UV detection wavelength was 282nm and 20μl sample was injected. The retention time for telmisartan was 3.32 min. The percentage RSD for precision and accuracy of the method was found to be less than 2. The method was validated as per the ICH guidelines. The method was successfully applied for routine analysis of telmisartan in tablet dosage form and in serum.
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تاریخ انتشار 2011